Samples and primers can be submitted in 1.5 ml tubes, strip tubes or 96 well PCR plates.
As for the DNA samples in water or TE, bacterial cells in agar-stab, or agar plate culture, temperature control is not necessary. Samples are stable for a few days at room temperature.
|
| a)
Sample for individual tube
: |
- General glycerol
stock/ Agar-stab/Agar-plate culture at room temperature.
- 1.5mL Microcentrifuge Tube is recommended in solution (Nuclease-free TE or distilled water) at room temperature.
- Free re-sequencing is included
|
| b)
96 Well Plate : |
- 8 strip cap is recommended in solution (Nuclease-free TE or distilled water) at room temperature.
- Re-sequencing is additionally charged.
** In case of Multi primers for one plate **
Only two custom primers (or two universal primers) in tubes could be acceptable
for applying this Plate sequencing.
* Note:
- For three or more primers, another paired primer plate with your sample….reactions.
- The well to well variation of the concentration of your samples is very important to get
better results, please keep it as constant as possible.
- Please prepare samples to avoid
any well-to-well concentration
difference or size differencefor
quality results.
|
| c) Primer : |
- Primer : 2-5 pmol/ul(uM) in 20µl based on
5 samples
- Primer Tm is suitable for service : 55°C ~ 60°C
- Commonly used Universal primers are available for free of charge
(ex. M13F, T7, T3)
- Design and synthesize primers if you request. (US $0.70/base)
|
|
| d) To apply Plate sequencing : |
- 1 primer/1plate service :
- Multi primers/ 1plate service :
Only
two custom primers (or two universal primers) in tubes could be acceptable
for applying this Plate
sequencing.
Note : For three or more primers, another paired primer plate with your sample
plate should be provided. If not, your order will be regarded as Normal sequencing reactions.
|
|
| - Sealing
of 96 Well Plates |
- Strip caps are recommended to seal 96-well
plates.
- Carefully seal the 96 well plates with strip caps (8-strips or 12-strips) or
polypropylene films
(relatively thick and malleable).
- Aluminum sealing or polyester films are not recommended.
Place samples into strip-capped well plate as shown below.
To avoid potential damage, please use out-skirted well plate.
Seal tightly to avoid sample evaporation
or contamination during transit.
Unsatisfactory results due to improper sample
preparation or shipping by customer will
be charged for re-sequencing. |
|
|
