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Macrogen Inc. offers DNA sequencing using state-of-the-art robotics and instrumentation to generate high quality sequence data at affordable prices. We have successfully sequenced countless samples of PCR products, plasmids, and BACs from clients all over the world, and our DNA sequencing services are highly rated for their turnaround time, quality of results, and competitive prices.





Performs all processes including nucleic acid extraction, PCR, sequencing, assembly using bacterial cells.
Deliver results within 7 business days from date of sample arrival
 
As shown in the figure below, living organisms can be classified into 3 primary lineages using the base sequence analysis of 16S rRNA: Domain Eucarya, Domain Bacteria, and Domain Archaea.



1Kb single contig : 5~6 days.
  (Time consuming would vary based on the following reasons; primer synthesis and sequencing
    failure)
Phred score of 20 or higher quality contig is requirerd.
Time can be saved when primers are synthesized bidirectionally.
  (refer to the figure as follows)
 
 
Starting with the end sequencing product by using the primer provided or designed,
  internal primer will be designed and processed.
 
Using a new primer (but with the same template), internal primer continues reading at
  the appropriate location.
 
Each walking requires approximately 5~6 days; enabling extension of 500bp~600bp per direction.
 

Number of primers and sequence used for primer walking

Abi file used for primer walking
Contig sequence and contig quality